Journal: Nutrients

Article Title: Hepatoprotective Effect of Antrodia camphorata Mycelium Powder on Alcohol-Induced Liver Damage

doi: 10.3390/nu16193406

Figure Lengend Snippet: Concentration of γ-GTP ( A ) and adiponectin ( B ) in the rat serum. Data were presented as the mean ± SE. The results were statistically analyzed using Student’s t -test or one-way ANOVA.

Article Snippet: The serum concentrations of γ-GTP (MBS9343646, Mybiosource, San Diego, CA, USA) and adiponectin (RRP300, R&D system, Minneapolis, MN, USA) were measured using ELISA kits once every two weeks.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells

doi: 10.3390/ijms25105155

Figure Lengend Snippet: Effects of eCG on ovarian and blood adiponectin secretion during hormone-induced ovarian development in rats. ( A ) The morphology of ovaries and uteri of rats in the eCG-treated and control groups; histological staining of ovaries from eCG-treated and control group rats. ( B ) Ovary weights and ovarian index of rats in the eCG-treated and control groups. ( C ) BrdU staining in rat ovaries from the eCG-treated and control groups and quantitative histograms. ( D ) Concentration of adiponectin in the ovaries of rats by ELISA. ( E ) Ovarian expression of adiponectin in rats from the eCG-treated and control groups. ( F ) Plasma adiponectin levels in rats from the eCG-treated and control groups. ( G ) Plasma glucose levels in rats from the eCG-treated and control groups. Scale bars: 500 μm. The error bars represent means ± SEM ( n = 3, each group). * Statistically significant values (**** p < 0.0001; *** p < 0.001; * p < 0.05) ns represent no significance. GC, granulosa cell; TC, theca cell; IC, interstitial cell; and O, oocyte.

Article Snippet: The concentrations of adiponectin in rat plasma and adiponectin in rat ovaries were measured using ELISA kits (CSB-E07271r for adiponectin, Cusabio Biotech Co., Wuhan, China) according to the protocol.

Techniques: Control, Staining, BrdU Staining, Concentration Assay, Enzyme-linked Immunosorbent Assay, Expressing, Clinical Proteomics

Journal: ACS Omega

Article Title: Oral Administration of the Adiponectin Receptor 1 Agonistic Dipeptide Tyr-Pro Prevents Hyperglycemia in Spontaneously Diabetic Torii Rats

doi: 10.1021/acsomega.4c09030

Figure Lengend Snippet: Involvement of Tyr-Pro in glucose uptake-related signaling pathways, AdipoR1-mediated Signaling Pathway, and insulin signaling pathway in SDT Rats. Plasma insulin (A) and adiponectin (B) levels in SDT rats euthanized at 29 weeks of age were measured using ELISA insulin/adiponectin assay kits. The protein expression of (C) AdipoR1, (D) p -AMPK/AMPK, (E) GLUT4, and (F) p -IRS-1(Tyr608)/IRS-1 and p -IRS-1(Ser612)/IRS-1 in skeletal muscle tissue of SDT rats euthanized at 29 weeks of age was measured using Wes analysis. Protein expression was normalized by the electropherogram peak area of total protein in each lane. The chemiluminescent signal was displayed as a virtual blot-like image, and an electropherogram was generated based on the molecular weight. Results are presented as the mean ± SEM ( n = 3–4). Statistical analyses between the two groups were evaluated by Student’s t -test at p < 0.05, p < 0.01, p < 0.001, and N . S .: not significant at p < 0.05.

Article Snippet: Plasma adiponectin levels were determined using a rat adiponectin ELISA kit (HMW adiponectin, Lot: 634–13071, FUJIFILM Wako Shibayagi Co.) according to the manufacturer’s instructions.

Techniques: Protein-Protein interactions, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Expressing, Generated, Molecular Weight